Core Curriculum

The Core Curriculum includes articles, images and online tutorials that are considered essential knowledge for all young investigators and LRRC members.

Articles

Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling
Rock JR, Randell SH, Hogan BL. Dis Model Mech. 2010 Sep-Oct;3(9-10):545-56

Conditional Control of Gene Expression in the Respiratory Epithelium
Jeffrey A. Whitsett and Anne-Karina T. Perl AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY VOL 34 2006

Conditional Depletion of Airway Progenitor Cells Induces Peribronchiolar Fibrosis
Anne-Karina T. Perl, Dieter Riethmacher, and Jeffrey A. Whitsett Am J Respir Crit Care Med Vol 183. pp 511–521, 2011

Conditional Expression of Genes in the Respiratory Epithelium in Transgenic Mice
Anne-Karina Perl, Liqian Zhang and Jeffrey A. Whitsett Am J Respir Cell Mol Biol Vol 40. pp 1–3, 2009

Directed Differentiation of Human Pluripotent Stem Cells into Intestinal Tissue in vitro
Jason R. Spence, Christopher N. Mayhew, Scott A. Rankin, Matthew Kuhar, Jefferson E. Vallance, Kathryn Tolle, Elizabeth E. Hoskins, Vladimir V. Kalinichenko, Susanne I. Wells, Aaron M. Zorn, Noah F. Shroyer, and James M. Wells Nature. 2011 February 3; 470(7332): 105–109

Directing Lung Endoderm Differentiation in Pluripotent Stem Cells
Kadzik RS, Morrisey EE Cell Stem Cell 2012 April 6; 10(4): 355-361

Dynamic Regulation of PDGFRα Expression in Alveolar fibroblasts During Realveolarization
Chen L, Acciani T, Le Cras T, Lutzko C, Perl AK. Am J Respir Cell Mol Biol. 2012 May 31.

Efficient Derivation of Purified Lung and Thyroid Progenitors from Embryonic Stem Cells
Longmire TA, Ikonomou L, Hawkins F, Christodoulou C, Cao Y, Jean JC, Kwok LW, Mou H, Rajagopal J, Shen SS, Dowton AA, Serra M, Weiss DJ, Green MD, Snoeck HW, Ramirez MI, Kotton DN. Cell Stem Cell. 2012 Apr 6;10(4):398-411.

Epithelial Progenitor Cells in Lung Development, Maintenance, Repair, and Disease
Jason R. Rock and Brigid L.M. Hogan Annual Review of Cell and Developmental Biology Vol. 27: 493-512

Evidence for type II cells as cells of origin of K-Ras-induced distal lung adenocarcinoma
Xu, X., Rock, J.R., Lu, Y., Futtner, C., Schwab, B. Guinney, J., Hogan, B.L.M and M.W Onaitis PNAS 109: 4910-4815 (2012)

Generation of Multipotent Lung and Airway Progenitors from Mouse ESCs and Patient-Specific Cystic Fibrosis iPSCs
Mou H, Zhao R, Sherwood R, Ahfeldt T, Lapey A, Wain J, Sicilian L, Izvolsky K, Musunuru K, Cowan C, Rajagopal J. Cell Stem Cell. 2012 Apr 6;10(4):385-97.

Multiple stromal cell populations contribute to pulmonary fibrosis without evidence for epithelial to mesenchymal transition
Rock, J.R., Barkauskas, C.E., Cronce, M.J., Xue, Y., Harris, J.R., Liang, J., Noble, P. and B.L.M. Hogan PNAS 108 E1475-83 (2012)

Preparing for the First Breath: Genetic and Cellular Mechanisms in Lung Development
Edward E. Morrisey, Brigid L.M. Hogan Developmental Cell, Volume 18, Issue 1, 8-23, 19 January 2010

Self-Renewing Endodermal Progenitor Lines Generated from Human Pluripotent Stem Cells
Cheng X, Ying L, Lu L, Galvão AM, Mills JA, Lin HC, Kotton DN, Shen SS, Nostro MC, Choi JK, Weiss MJ, French DL, Gadue P. Cell Stem Cell. 2012 Apr 6;10(4):371-84.

The a“MAZE”ing World of Lung-Specific Transgenic Mice
Emma L. Rawlins and Anne-Karina Perl Am J Respir Cell Mol Biol Vol 46, Iss. 3, pp 269–282, Mar 2012

Transcriptional Control of Lung Morphogenesis
YUTAKA MAEDA, VRUSHANK DAVÉ, AND JEFFREY A. WHITSETT Physiol Rev 87: 219–244, 2007

Images

bluelung

Section lung from Foxj1-GFP E15 embryo showing differentiation of future multiciliated cells in the developing airways but not in the branching tips

Credit: Emma Rawlins

Reference: Rock et al Epithelial Progenitor Cells in lung development, maintenance, repair and disease Ann. Rev Cell Dev Biol. (2011) 27: 493-51

purplelung

Section of E14.5 embryonic mouse lung showing differentiation of Foxj1-GFP + cells (marking future multiciliated cells) in the more proximal airways and expression of Sox9 (red) in the undifferentiated mutipotent distal tip cells Nuclei stained with DAPI

Credit: Emma Rawlins (Gurdon Institute Cambridge)

greenlung

Intact lungs from E12.5 Sftpc-GFP transgenic mouse line showing transgene expression in the distal tips of the endodermal buds

Credit: This line was made by John Heath University of Manchester UK (J.K.Heath@bham.ac.uk)

whitelung

Scanning EM of a section through the adult mouse lung.

Credit: Taken from a “Lung Tour” on line from the Lawrence Berkeley National lab which starts at http://imglib.lbl.gov/ImgLib/COLLECTIONS/LUNG_STRUCTURE/.tour/page1.html

redlung

Vibrotome section of mouse E16.5 embryonic lung immunostained with antibody to Sox2 (green) and Sox9 (red) showing proximal-distal patterning of the lung endoderm at this stage

Credit: Emma Rawlins, Gurdon Institute Cambridge UK (e.rawlins@gurdon.cam.ac.uk)

purplelung2

Vibrotome section of E16 embryonic mouse lung immunostained with antibodies to e-cadherin (epithelial cells, green), PeCAM/CD31 (endothelial cells, pink) and acetylated a-tubulin (cilia, red) and DAPI (blue, nuclei)

Credit: Emma Rawlins

Reference: Rock et al Epithelial Progenitor Cells in lung development, maintenance, repair and disease Ann. Rev Cell Dev Biol. (2011) 27: 493-512

Tutorials

lungthing

Procedure for Lung Engineering
Elizabeth A. Calle, Thomas H. Petersen, Laura E. Niklason